Human TNFRSF9/4-1BB ELISA Kit PicoKine®

Mfr.No.

EK0988

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.01	0.091	0.171	0.35	0.627	1.05	1.66	2.217

Description

Human TNFRSF9/4-1BB ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF9 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 9;4-1BB ligand receptor;CDw137;T-cell antigen 4-1BB homolog;T-cell antigen ILA;CD137;TNFRSF9;CD137, ILA;

Molecular Weight

27899 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-Q186

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF9

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	70	317	916	64	324	950
Standard deviation	7.5%5	23.45	50.38	5.44	30.13	56.05
CV (%)	7.5%	7.4%	5.5%	8.5%	9.3%	5.9%

Protein Function

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Background

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alteative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	70	70	78	74	73	3.31	4.5%
Sample 2	317	278	269	308	293	20.01	6.8%
Sample 3	916	936	953	893	924	22.41	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3604
Gene Name	TNFRSF9
UniProt ID	Q07011
Protein Name	Tumor necrosis factor receptor superfamily member 9
Tissue Specificity	Expressed on the surface of activated T-cells.
Sequence	Contains 4 TNFR-Cys repeats.

Applications	ELISA
Research Area	Stem Cells,T Lymphocytic Lineage,TNF Superfamily,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000751	EK0988	96 wells/kit	$499		Add to Cart

Human TNFRSF9/4-1BB ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human TNFRSF9/4-1BB ELISA Kit PicoKine®

Rate it now..!!