Human TNFRSF25/DR3 ELISA Kit PicoKine®

Mfr.No.

EK1579

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.047	0.117	0.148	0.336	0.429	0.724	1.255	2.087

Description

Human TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF25 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 25;Apo-3;Apoptosis-inducing receptor AIR;Apoptosis-mediating receptor DR3;Apoptosis-mediating receptor TRAMP;Death receptor 3;Lymphocyte-associated receptor of death;LARD;Protein WSL;Protein WSL-1;TNFRSF25;APO3, DDR3, DR3, TNFRSF12, WSL, WSL1;UNQ455/PRO779;

Molecular Weight

45385 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-F201

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF25

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	691	2487	9783	668	2430	10603
Standard deviation	37.31	154.19	489.15	42.75	196.83	689.19
CV (%)	5.4%	6.2%	5%	6.4%	8.1%	6.5%

Protein Function

Receptor for TNFSF12/APO3L/TWEAK. Interacts ly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.

Background

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alteatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alteative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	691	639	639	669	659	21.92	3.3%
Sample 2	2487	2310	2253	2294	2336	89.62	3.8%
Sample 3	9783	10046	9881	10103	9953	127.7	1.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8718
Gene Name	TNFRSF25
UniProt ID	Q93038
Protein Name	Tumor necrosis factor receptor superfamily member 25
Tissue Specificity	Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.

Applications	ELISA
Research Area	Apoptosis,Cell Biology,NFKB Pathway,Nuclear Signaling,Receptors,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001264	EK1579	96 wells/kit	$750		Add to Cart

Human TNFRSF25/DR3 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human TNFRSF25/DR3 ELISA Kit PicoKine®

Rate it now..!!