Human TNFRSF17/BCMA ELISA Kit PicoKine®

Mfr.No.

EK0661

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.011	0.154	0.256	0.452	0.734	1.225	1.678	1.984

Description

Human TNFRSF17/BCMA ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF17 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 17;B-cell maturation protein;CD269;TNFRSF17;BCM, BCMA;

Molecular Weight

20165 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A54

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF17

Subcellular Localization

Cell membrane; Single-pass type III membrane protein. Endomembrane system; Single-pass type III membrane protein. Perinuclear Golgi-like structures.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	64	237	917	68	247	851
Standard deviation	4.09	13.98	42.18	5.57	16.54	43.4
CV (%)	6.4%	4.6%	4.6%	8.2%	6.7%	5.1%

Protein Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. .

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	64	58	60	62	61	2.23	3.6%
Sample 2	237	213	221	231	225	9.2	4%
Sample 3	917	840	916	821	873	43.52	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	608
Gene Name	TNFRSF17
UniProt ID	Q02223
Protein Name	Tumor necrosis factor receptor superfamily member 17
Tissue Specificity	Expressed in mature B-cells, but not in T- cells or monocytes.
Sequence	Contains 1 TNFR-Cys repeat.

Applications	ELISA
Research Area	Antiviral Signaling,Cancer,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000550	EK0661	96 wells/kit	$499		Add to Cart

Human TNFRSF17/BCMA ELISA Kit PicoKine®

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