Human TNFRSF17/BCMA ELISA Kit PicoKine®

Mfr.No.

EK0661

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.011	0.154	0.256	0.452	0.734	1.225	1.678	1.984

Description

Human TNFRSF17/BCMA ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 17;B-cell maturation protein;CD269;TNFRSF17;BCM, BCMA;

Molecular Weight

20165 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A54

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TNFRSF17

Subcellular Localization

Cell membrane; Single-pass type III membrane protein. Endomembrane system; Single-pass type III membrane protein. Perinuclear Golgi-like structures.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	64	237	917	68	247	851
Standard deviation	4.09	13.98	42.18	5.57	16.54	43.4
CV (%)	6.4%	4.6%	4.6%	8.2%	6.7%	5.1%

Protein Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. .

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	64	58	60	62	61	2.23	3.6%
Sample 2	237	213	221	231	225	9.2	4%
Sample 3	917	840	916	821	873	43.52	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	608
Antigen	TNFRSF17/BCMA
Gene Name	TNFRSF17
UniProt ID	Q02223
Protein Name	Tumor necrosis factor receptor superfamily member 17
Tissue Specificity	Expressed in mature B-cells, but not in T- cells or monocytes.
Sequence	Contains 1 TNFR-Cys repeat.

Applications	ELISA
Research Area	Antiviral Signaling,Cancer,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000550	EK0661	96 wells/kit	$499		Add to Cart

Human TNFRSF17/BCMA ELISA Kit PicoKine®

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