Human Tissue Factor Pathway Inhibitor

Mfr.No.

E1188Hu

Description

This sandwich kit is for the accurate quantitative detection of human Tissue Factor Pathway Inhibitor (also known as TFPI) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EPI;Extrinsic pathway inhibitor;LACI;Lipoprotein-associated coagulation inhibitor;TFI;TFPI;TFPI1;Tissue factor pathway inhibitor

Reactivity

Human

Detection Range

5-900ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

35015

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.02ng/ml

Background

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Gene ID	7035
UniProt ID	P10646
Antigen	TFPI

Research Area

Cardiovascular

Protocol

These standard curves of E1188Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/ml	2.116	2.167	2.105
	2.218
240ng/ml	1.539	1.442	1.38
	1.345
120ng/ml	0.943	0.859	0.797
	0.775
60ng/ml	0.432	0.491	0.429
	0.551
30ng/ml	0.253	0.236	0.173
	0.218
0ng/ml	0.068	0.062	0
	0.057

Precision

We measured random samples of E1188Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	330.45	9.39	2.84
Intra-Assay	2	18	81.82	4.7	5.74
Intra-Assay	3	18	114.49	5.44	4.76

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102835	E1188Hu	48T	$320		Add to Cart
BHE12102835	E1188Hu	96T	$458		Add to Cart

Human Tissue Factor Pathway Inhibitor - TFPI ELISA Kit

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