Human TIMP-4 ELISA Kit PicoKine®

Mfr.No.

EK0524

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.006	0.109	0.232	0.519	0.998	1.381	1.809	2.166

Description

Human TIMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human TIMP4 in cell culture supeatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;TIMP4;

Molecular Weight

25503 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C30-P224

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	123	589	2056	122	601	1963
Standard deviation	9.47	38.87	125.41	10.98	48.08	155.07
CV (%)	7.7%	6.6%	6.1%	9%	8%	7.9%

Protein Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9.

Background

Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	123	131	119	122	123	4.43	3.6%
Sample 2	589	618	605	599	602	10.49	1.7%
Sample 3	2056	2211	2029	2343	2159	126.55	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7079
Gene Name	TIMP4
UniProt ID	Q99727
Protein Name	Metalloproteinase inhibitor 4
Tissue Specificity	Abundant in heart and present at low levels in many other tissues.
Sequence	Belongs to the protease inhibitor I35 (TIMP) family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Cell Biology,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis/Ubiquitin

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000497	EK0524	96 wells/kit	$499		Add to Cart

Human TIMP-4 ELISA Kit PicoKine®

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