Human Thymidylate Synthetase

Mfr.No.

E0770Hu

Description

This sandwich kit is for the accurate quantitative detection of human Thymidylate Synthetase (also known as TS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HST422;Thymidylate Synthase;TMS;TS;TSase

Reactivity

Human

Detection Range

0.2-60mg/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

35716

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.11mg/ml

Background

Thymidylate synthase (TS) (EC 2.1.1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise. Both cause DNA damage.

Gene ID	7298
UniProt ID	P04818
Antigen	TS

Research Area

Others

Protocol

These standard curves of E0770Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40mg/ml	2.172	2.288	2.256
	2.403
20mg/ml	1.377	1.282	1.25
	1.186
10mg/ml	0.639	0.675	0.644
	0.712
5mg/ml	0.352	0.323	0.291
	0.293
2.5mg/ml	0.149	0.182	0.15
	0.215
0mg/ml	0.04	0.032	0
	0.024

Precision

We measured random samples of E0770Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.3	0.62	3.05
Intra-Assay	2	18	13.45	0.6	4.47
Intra-Assay	3	18	5.46	0.3	5.43

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102449	E0770Hu	48T	$320		Add to Cart
BHE12102449	E0770Hu	96T	$458		Add to Cart

Human Thymidylate Synthetase - TS ELISA Kit

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