Human Thymidinephosphorylase

Mfr.No.

E0771Hu

Sensitivity

0.16ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Thymidinephosphorylase (also known as TP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Gliostatin;PD-ECGF;Platelet-derived endothelial cell growth factor;TdRPase;Thymidine phosphorylase;TP;TYMP

Molecular Weight

49955

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0771Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	31	1.38	4.4
Intra-Assay	2	18	19.6	0.81	4.1
Intra-Assay	3	18	7	0.32	4.6

Background

May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Protocol

These standard curves of E0771Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.16	2.062	2.014
	1.963
24ng/ml	1.447	1.417	1.369
	1.388
12ng/ml	0.624	0.648	0.6
	0.672
6ng/ml	0.355	0.382	0.334
	0.408
3ng/ml	0.147	0.177	0.129
	0.208
0ng/ml	0.043	0.048	0
	0.053

Gene ID	1890
UniProt ID	P19971

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102450	E0771Hu	96T	$458		Add to Cart
BHE12102450	E0771Hu	48T	$320		Add to Cart

Human Thymidinephosphorylase - TP ELISA Kit

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