Human Thrombospondin 1 - TSP-1 ELISA Kit

Mfr.No.

E1110Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Thrombospondin 1 (also known as TSP-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Glycoprotein G;THBS 1;THBS1;Thrombospondin-1

Reactivity

Human

Detection Range

5-700ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

129383

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.39ng/ml

Background

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).

Gene ID	7057
UniProt ID	P07996
Antigen	THBS1

Research Area

Cardiovascular

Protocol

These standard curves of E1110Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
360ng/ml	2.077	2.066	2.027
	2.055
180ng/ml	1.446	1.484	1.444
	1.521
90ng/ml	0.68	0.729	0.69
	0.779
45ng/ml	0.369	0.411	0.372
	0.454
22.5ng/ml	0.121	0.152	0.112
	0.182
0ng/ml	0.039	0.039	0
	0.04

Precision

We measured random samples of E1110Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	219.6	9.66	4.4
Intra-Assay	2	18	69.2	2.83	4.1
Intra-Assay	3	18	140	7.59	5.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102758	E1110Hu	48T	$320		Add to Cart
BHE12102758	E1110Hu	96T	$458		Add to Cart

Human Thrombospondin 1 - TSP-1 ELISA Kit

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