Human Thrombomodulin - TM ELISA Kit

Mfr.No.

E1183Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Thrombomodulin (also known as TM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD141;Fetomodulin;THBD;Thrombomodulin;TM

Molecular Weight

60329

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1183Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.5	0.16	6.6
Intra-Assay	2	18	3.6	0.17	4.6
Intra-Assay	3	18	7.5	0.25	3.3

Background

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Protocol

These standard curves of E1183Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.192	2.213	2.172
	2.235
6ng/ml	1.184	1.24	1.199
	1.296
3ng/ml	0.718	0.771	0.729
	0.824
1.5ng/ml	0.377	0.415	0.373
	0.452
0.75ng/ml	0.223	0.226	0.185
	0.23
0ng/ml	0.026	0.042	0
	0.058

Gene ID	7056
UniProt ID	P07204

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102830	E1183Hu	48T	$320		Add to Cart
BHE12102830	E1183Hu	96T	$458		Add to Cart

Human Thrombomodulin - TM ELISA Kit

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