Human Thiamin Pyrophosphokinase 1

Mfr.No.

E6264Hu

Sensitivity

9.53ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

hTPK1;Placental protein 20;PP20;Thiamin pyrophosphokinase 1;Thiamine pyrophosphokinase 1;TPK 1;TPK1

Molecular Weight

27265

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6264Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	245.5	12.67	5.2
Intra-Assay	2	18	611.5	39.33	6.4
Intra-Assay	3	18	1117.9	67.77	6.1

Background

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Protocol

These standard curves of E6264Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.361	2.273	2.228
	2.186
1200ng/L	1.891	1.858	1.813
	1.824
600ng/L	1.318	1.222	1.177
	1.126
300ng/L	0.606	0.624	0.579
	0.642
150ng/L	0.35	0.371	0.326
	0.392
0ng/L	0.047	0.045	0
	0.043

Gene ID	27010
UniProt ID	Q9H3S4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107626	E6264Hu	48T	$320		Add to Cart
BHE12107626	E6264Hu	96T	$458		Add to Cart

Human Thiamin Pyrophosphokinase 1 - TPK1 ELISA Kit

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