Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®

Mfr.No.

EK0899

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.116	0.225	0.297	0.401	0.747	1.194	1.693	2.074

Description

Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Thrombospondin-1;THBS1;TSP, TSP1;

Molecular Weight

129383 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: N19-P1170

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human THBS1

Subcellular Localization

Endoplasmic reticulum . Sarcoplasmic reticulum .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2414	13444	38908	2523	13902	38494
Standard deviation	152.08	994.85	2451.2	176.61	1278.98	2771.56
CV (%)	9.2%%	7.4%	9.2%%	7%	9.2%	7.2%

Protein Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). .

Background

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2414	2383	2098	2136	2257	141.81	6.2%
Sample 2	13444	12251	11532	12803	12507	703.87	5.6%
Sample 3	38908	44108	38626	45595	41809	3088.95	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7057
Antigen	THBS1/TSP1/Thrombospondin-1
Gene Name	THBS1
UniProt ID	P07996
Protein Name	Thrombospondin-1
Sequence	Belongs to the thrombospondin family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Cell Adhesion Proteins,Cell Type Marker,Invasion/Microenvironment,Metabolism,Metabolism Processes,Neuroscience,Pathways and Processes,Platelets,Response To Hypoxia,Secreted,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000681	EK0899	96 wells/kit	$499		Add to Cart

Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®

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