Human Tgfb1-induced Anti-Apoptotic Factor 1

Mfr.No.

E6263Hu

Alternative Names

12 kDa TGF-beta-1-induced antiapoptotic factor;TGFB1-induced anti-apoptotic factor 1;TIAF 1;TIAF1

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

12414

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.28ng/L

Background

Inhibits the cytotoxic effects of TNF-alpha and overexpressed TNF receptor adapters TRADD, FADD, and RIPK1. Involved in TGF-beta1 inhibition of IkappaB-alpha expression and suppression of TNF-mediated IkappaB-alpha degradation.

Gene ID	9220
UniProt ID	O95411
Antigen	TIAF1

Research Area

Others

Protocol

These standard curves of E6263Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.956	1.912	1.868
	1.868
600ng/L	0.991	0.991	0.948
	0.991
300ng/L	0.465	0.447	0.403
	0.428
150ng/L	0.283	0.241	0.197
	0.199
75ng/L	0.088	0.114	0.07
	0.139
0ng/L	0.055	0.044	0
	0.033

Precision

We measured random samples of E6263Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	681.9	26.6	3.9
Intra-Assay	2	18	826	21.62	2.6
Intra-Assay	3	18	304.1	16.98	5.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107625	E6263Hu	48T	$320		Add to Cart
BHE12107625	E6263Hu	96T	$458		Add to Cart

Human Tgfb1-induced Anti-Apoptotic Factor 1 - TIAF1 ELISA Kit

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