Human Tetranectin/CLEC3B ELISA Kit PicoKine®

Mfr.No.

EK1253

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.023	0.093	0.158	0.271	0.504	0.878	1.344	1.938

Description

Human Tetranectin/CLEC3B ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC3B in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tetranectin;TN;C-type lectin domain family 3 member B;Plasminogen kringle 4-binding protein;CLEC3B;TNA;

Molecular Weight

22537 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V202

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Tetranectin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	427	2683	8208	405	2547	7804
Standard deviation	28.6	107.32	4632.45	32.4	150.27	561.88
CV (%)	6.7%	4%	5.7%	8%	5.9%	7.2%

Protein Function

Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.

Background

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	427	481	500	446	463	28.62	6.1%
Sample 2	2683	2751	2924	3083	2860	155.75	5.4%
Sample 3	8208	9087	8514	7745	8388	487.42	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7123
Gene Name	CLEC3B
UniProt ID	P05452
Protein Name	Tetranectin
Tissue Specificity	Found in plasma.
Sequence	Contains 1 C-type lectin domain.

Applications	ELISA
Research Area	Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Serum Proteins,Signal Transduction,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000853	EK1253	96 wells/kit	$499		Add to Cart

Human Tetranectin/CLEC3B ELISA Kit PicoKine®

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