Human Teratocarcinoma Derived Growth Factor 1

Mfr.No.

E2603Hu

Description

This sandwich kit is for the accurate quantitative detection of human Teratocarcinoma Derived Growth Factor 1 (also known as TDGF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CRGF;Cripto-1 growth factor;Epidermal growth factor-like cripto protein CR1;TDGF 1;TDGF1;Teratocarcinoma-derived growth factor 1

Reactivity

Human

Detection Range

30-9000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21169

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

13.96ng/L

Background

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed: 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed: 11909953).

Gene ID	6997
UniProt ID	P13385
Antigen	TDGF1

Research Area

Others

Protocol

These standard curves of E2603Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.272	2.24	2.206
	2.208
2400ng/L	1.185	1.3	1.267
	1.416
1200ng/L	0.597	0.622	0.589
	0.648
600ng/L	0.329	0.342	0.309
	0.355
300ng/L	0.123	0.164	0.13
	0.204
0ng/L	0.015	0.034	0
	0.052

Precision

We measured random samples of E2603Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2279.5	122.75	5.4
Intra-Assay	2	18	3548.4	130.38	3.7
Intra-Assay	3	18	1225.4	41.13	3.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104141	E2603Hu	48T	$320		Add to Cart
BHE12104141	E2603Hu	96T	$458		Add to Cart

Human Teratocarcinoma Derived Growth Factor 1 - TDGF1 ELISA Kit

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