Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

Mfr.No.

EK2038

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.015	0.129	0.223	0.423	0.689	1.097	1.676	2.111

Description

Human TCN2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TCN2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-W427

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TCN2

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	285	1139	5792	1123	1123	5755
Standard deviation	17.38	72.89	457.56	18.45	74.11	552.48
CV (%)	6.1%	6.4%	7.9%	6.6%	6.6%	9.6%

Protein Function

Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.

Background

TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	285	292	319	286	295	13.82	4.6%
Sample 2	1139	1247	1212	1220	1204	39.97	3.3%
Sample 3	5792	6174	5259	6098	5830	359.73	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6948
Antigen	TCN2/Transcobalamin-2
Gene Name	TCN2
UniProt ID	P20062
Protein Name	Transcobalamin-2

Applications	ELISA
Research Area	Cardiovascular,Cofactors, Vitamins/Minerals,Metabolism,Pathways and Processes,Serum Proteins,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001533	EK2038	96 wells/kit	$750		Add to Cart

Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

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