Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

Mfr.No.

EK2038

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.015	0.129	0.223	0.423	0.689	1.097	1.676	2.111

Description

Human TCN2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TCN2 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-W427

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TCN2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	285	1139	5792	1123	1123	5755
Standard deviation	17.38	72.89	457.56	18.45	74.11	552.48
CV (%)	6.1%	6.4%	7.9%	6.6%	6.6%	9.6%

Protein Function

Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.

Background

TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alteatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	285	292	319	286	295	13.82	4.6%
Sample 2	1139	1247	1212	1220	1204	39.97	3.3%
Sample 3	5792	6174	5259	6098	5830	359.73	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6948
Gene Name	TCN2
UniProt ID	P20062
Protein Name	Transcobalamin-2

Applications	ELISA
Research Area	Cardiovascular,Cofactors, Vitamins/Minerals,Metabolism,Pathways and Processes,Serum Proteins,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001533	EK2038	96 wells/kit	$750		Add to Cart

Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

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