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Home / ELISA Kit

Human Tapasin (TAPBP) ELISA Kit

SKU : BHE13702690

TAPBP encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and TAPBP may be bound to a single TAP molecule. Tapasin contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.

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Mfr.No.	KTE60473
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tapasin (TAPBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPBP.
Alternative Names	TAPBP; DADB-159G18.7; NGS17; TAPA; TPN; TPSN; TAP-associated protein; tapasin
Detection Method	Colorimetric
Kit Components	Human Tapasin microplate Human Tapasin standard Human Tapasin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tapasin (TAPBP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAPBP. No significant cross-reactivity or interference between Human TAPBP and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TAPBP encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and TAPBP may be bound to a single TAP molecule. Tapasin contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alteative splicing results in three transcript variants encoding different isoforms.
Notes	This Human Tapasin (TAPBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPBP in samples. An antibody specific for TAPBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6892

Applications	ELISA
Applications Notes	This Human Tapasin (TAPBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPBP in samples. An antibody specific for TAPBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702690	KTE60473	48 T	$339		Add to Cart
BHE13702690	KTE60473	96 T	$589		Add to Cart
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BHE13702690	KTE60473	96 Tx50	$24569		Add to Cart

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