Human TAFI/CPB2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1502

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.056	0.137	0.227	0.314	0.583	1.091	1.693	2.429

Description

Human TAFI/CPB2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CPB2 in cell culture supeatants, serum and plasma (heparin, EDTA).

Alternative Names

Carboxypeptidase B2;3.4.17.20 ;Carboxypeptidase U;CPU;Plasma carboxypeptidase B;pCPB;Thrombin-activable fibrinolysis inhibitor;TAFI;CPB2;

Molecular Weight

48424 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-V423

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	752	2639	6784	886	2947	7253
Standard deviation	38.35	139.86	434.17	57.59	221.02	594.74
CV (%)	5.1%	5.3%	6.4%	6.5%	7.5%	8.2%

Protein Function

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .

Background

Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	1361
Gene Name	CPB2
UniProt ID	Q96IY4
Protein Name	Carboxypeptidase B2
Tissue Specificity	Plasma; synthesized in the liver.

Applications	ELISA
Research Area	Amino Acid Metabolism,Amino Acids,Atherosclerosis,Cardiovascular,Heart Disease,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Serum Proteins,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000027	EZ1502	5 plates/kit	$500		Add to Cart

Human TAFI/CPB2 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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