Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Mfr.No.

EK1356

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.042	0.077	0.159	0.261	0.404	0.800	1.503	1.872

Description

Human Syndecan-4/SDC4 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC4 in cell culture supeatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4;

Molecular Weight

21642 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-E145

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Syndecan-4

Subcellular Localization

Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form. .

Sample Type

Cell culture supeatants, Cell lysates, Serum

Anticoagulant

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	94	673	2108	102	680	1958
Standard deviation	3.85	52.49	128.58	5.4	56.44	154.68
CV (%)	4.1%	7.8%	6.1%	5.3%	8.3%	7.9%

Protein Function

Cell surface proteoglycan that bears heparan sulfate.

Background

Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. Whats more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	94	82	81	78	83	6.09	7.3%
Sample 2	673	735	621	630	664	45.06	6.7%
Sample 3	2108	2092	1869	1903	1993	107.82	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6385
Gene Name	SDC4
UniProt ID	P31431
Protein Name	Syndecan-4
Tissue Specificity	Expressed in epithelial and fibroblastic cells. .

Applications	ELISA
Research Area	Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Proteins,Neurogenesis,Neurology Process,Neuroscience,Plasma Membrane,Signal Transduction,Subcellular Markers,Tags & Cell Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000950	EK1356	96 wells/kit	$499		Add to Cart

Human Syndecan-4/SDC4 ELISA Kit PicoKine®

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