Human Synaptosomal-associated Protein 25

Mfr.No.

E3674Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Synaptosomal-Associated Protein 25 (also known as SNAP25) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

bA416N4.2;CMS18;dJ1068F16.2;RIC4;RIC-4;SEC9;SNAP;SNAP25;SNAP-25;SUP;Super protein;Synaptosomal-associated 25 kDa protein;Synaptosomal-associated protein 25;

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

23315

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/ml

Background

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Gene ID	6616
UniProt ID	P60880
Antigen	SNAP25

Research Area

Neuroscience

Protocol

These standard curves of E3674Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.331	2.266	2.216
	2.202
6ng/ml	1.345	1.416	1.366
	1.487
3ng/ml	0.797	0.862	0.812
	0.928
1.5ng/ml	0.458	0.454	0.404
	0.451
0.75ng/ml	0.269	0.268	0.218
	0.268
0ng/ml	0.056	0.05	0
	0.044

Precision

We measured random samples of E3674Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.16	0.15	4.78
Intra-Assay	2	18	5.61	0.19	3.33
Intra-Assay	3	18	1.42	0.1	6.87

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105148	E3674Hu	48T	$320		Add to Cart
BHE12105148	E3674Hu	96T	$458		Add to Cart

Human Synaptosomal-associated Protein 25 - SNAP25 ELISA Kit

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