Human suppressors of cytokine signaling 3,SOCS-3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11637h

Sensitivity

11.75 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

suppressor of cytokine signaling 3, ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, STAT induced STAT inhibitor 3, cytokine-induced SH2 protein 3

Recovery

The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	103	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	92
	Range %	88-94
1:2000	Average %	93
	Range %	90-98
1:4000	Average %	88
	Range %	85-94
1:8000	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O14543
Tissue Specificity	Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Applications	ELISA
Research Area	SOCS3

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504467	CSB-E11637h	10x96T	$6602.50	Add to Cart