Human Succinate Dehydrogenase Iron-sulfur Subunit - Mitochondrial

Mfr.No.

E3726Hu

Description

This sandwich kit is for the accurate quantitative detection of human Succinate Dehydrogenase[ubiquinone]Iron-sulfur Subunit; Mitochondrial (also known as SDHB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CWS2;IP;Iron-sulfur subunit of complex II;MC2DN4;PGL4;SDH;SDH1;SDH2;SDHB;Succinate dehydrogenase (ubiquinone) iron-sulfur subunit, mitochondrial

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

31630

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.027ng/ml

Background

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

Gene ID	6390
UniProt ID	P21912
Antigen	SDHB

Research Area

Others

Protocol

These standard curves of E3726Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.16	2.231	2.189
	2.303
10ng/ml	1.368	1.345	1.302
	1.321
5ng/ml	0.779	0.787	0.744
	0.794
2.5ng/ml	0.501	0.444	0.401
	0.387
1.25ng/ml	0.228	0.2	0.158
	0.172
0ng/ml	0.047	0.043	0
	0.038

Precision

We measured random samples of E3726Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.22	0.28	5.38
Intra-Assay	2	18	12.79	0.47	3.67
Intra-Assay	3	18	3.21	0.18	5.71

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105189	E3726Hu	48T	$320		Add to Cart
BHE12105189	E3726Hu	96T	$458		Add to Cart

Human Succinate Dehydrogenase Iron-sulfur Subunit - Mitochondrial - SDHB ELISA Kit

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