Human Structural Maintenance of Chromosomes Protein 3

Mfr.No.

E3560Hu

Description

This sandwich kit is for the accurate quantitative detection of human Structural Maintenance of Chromosomes Protein 3 (also known as SMC3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Bamacan;Basement membrane-associated chondroitin proteoglycan;Chondroitin sulfate proteoglycan 6;Chromosome-associated polypeptide;hCAP;SMC 3;SMC protein 3;SMC3;SMC-3;Structural maintenance of chromosomes protein 3

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

141542

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/ml

Background

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Gene ID	9126
UniProt ID	Q9UQE7
Antigen	SMC3

Research Area

Others

Protocol

These standard curves of E3560Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.415	2.364	2.322
	2.313
4ng/ml	1.284	1.362	1.32
	1.441
2ng/ml	0.725	0.731	0.689
	0.737
1ng/ml	0.447	0.473	0.432
	0.499
0.5ng/ml	0.305	0.299	0.257
	0.293
0ng/ml	0.041	0.042	0
	0.043

Precision

We measured random samples of E3560Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2	0.14	6.7
Intra-Assay	2	18	5.7	0.2	3.5
Intra-Assay	3	18	1.2	0.07	5.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105042	E3560Hu	48T	$320		Add to Cart
BHE12105042	E3560Hu	96T	$458		Add to Cart

Human Structural Maintenance of Chromosomes Protein 3 - SMC3 ELISA Kit

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