Human Stress-70 Protein - Mitochondrial

Mfr.No.

E3017Hu

Sensitivity

0.019ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

75 kDa glucose-regulated protein;CRP40;CSA;EVPLS;GRP-75;Heat shock 70 kDa protein 9;HEL-S-124m;HSPA9;HSPA9B;Mortalin;MOT;MOT2;MTHSP75;PBP74;Peptide-binding protein 74;SAAN;SIDBA4;Stress-70 protein, mitochondrial

Molecular Weight

73680

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3017Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.87	0.2	5.24
Intra-Assay	2	18	5.23	0.19	3.63
Intra-Assay	3	18	2.26	0.11	4.76

Background

Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed:26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed:21123823, PubMed:26702583). May play a role in the control of cell proliferation and cellular aging (By similarity).

Protocol

These standard curves of E3017Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.017	2.02	1.994
	2.022
6ng/ml	1.351	1.379	1.354
	1.407
3ng/ml	0.731	0.734	0.709
	0.737
1.5ng/ml	0.411	0.427	0.402
	0.443
0.75ng/ml	0.145	0.134	0.108
	0.122
0ng/ml	0.023	0.025	0
	0.028

Gene ID	3313
UniProt ID	P38646

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104554	E3017Hu	48T	$320		Add to Cart
BHE12104554	E3017Hu	96T	$458		Add to Cart

Human Stress-70 Protein - Mitochondrial - HSPA9 ELISA Kit

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