Human Steroid Hormone Receptor Err1

Mfr.No.

E6223Hu

Alternative Names

ERR1;ERRa;ERRalpha;ERR-alpha;ESRL1;ESRRA;Estrogen receptor-like 1;Estrogen-related receptor alpha;NR3B1;Nuclear receptor subfamily 3 group B member 1;Steroid hormone receptor ERR1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

45510

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.84ng/L

Background

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

Gene ID	2101
UniProt ID	P11474
Antigen	ESRRA

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E6223Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.331	2.482	2.421
	2.633
1200ng/L	1.505	1.507	1.446
	1.509
600ng/L	0.929	0.898	0.837
	0.867
300ng/L	0.465	0.493	0.432
	0.52
150ng/L	0.266	0.256	0.194
	0.245
0ng/L	0.056	0.061	0
	0.066

Precision

We measured random samples of E6223Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1134.19	29.58	2.61
Intra-Assay	2	18	676.17	36.2	5.35
Intra-Assay	3	18	501.77	28.99	5.78

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107585	E6223Hu	48T	$320		Add to Cart
BHE12107585	E6223Hu	96T	$458		Add to Cart

Human Steroid Hormone Receptor Err1 - ESRRA ELISA Kit

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