Human Stanniocalcin 1 / STC1 / STC ELISA Kit PicoKine®

Mfr.No.

EK1404

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.034	0.073	0.125	0.188	0.344	0.539	1.009	1.895

Description

Human Stanniocalcin 1 / STC1 / STC ELISA Kit PicoKine® (96 Tests). Quantitate Human STC1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Stanniocalcin-1;STC-1;STC1;STC;

Molecular Weight

27621 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V34-A247

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human STC1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	75	279	1061	71	306	1123
Standard deviation	3.9	13.11	56.23	4.04	19.27	60.64
CV (%)	5.2%	4.7%	5.3%	5.7%	6.3%	5.4%

Protein Function

Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.

Background

Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	75	70	70	78	73	3.41	4.6%
Sample 2	279	279	267	314	284	17.58	6.1%
Sample 3	1061	980	888	961	972	61.56	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6781
Gene Name	STC1
UniProt ID	P52823
Protein Name	Stanniocalcin-1
Tissue Specificity	Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.

Applications	ELISA
Research Area	Cancer,Cell Biology,Cofactors, Vitamins/Minerals,Growth Factors/Hormones,Metabolism,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001000	EK1404	96 wells/kit	$450		Add to Cart

Human Stanniocalcin 1 / STC1 / STC ELISA Kit PicoKine®

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