Human Spondin-1 - SPON1 ELISA Kit

Mfr.No.

E6219Hu

Sensitivity

0.028ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

F-spondin;SPON 1;SPON1;Spondin-1;Vascular smooth muscle cell growth-promoting factor

Molecular Weight

90973

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6219Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.7	0.1	3.8
Intra-Assay	2	18	7.8	0.35	4.5
Intra-Assay	3	18	4.5	0.22	4.9

Background

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.

Protocol

These standard curves of E6219Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	1.973	1.982	1.939
	1.991
6ng/mL	1.513	1.434	1.39
	1.354
3ng/mL	0.564	0.616	0.573
	0.668
1.5ng/mL	0.389	0.376	0.333
	0.362
0.75ng/mL	0.132	0.152	0.109
	0.172
0ng/mL	0.041	0.043	0
	0.045

Gene ID	10418
UniProt ID	Q9HCB6

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107581	E6219Hu	48T	$320		Add to Cart
BHE12107581	E6219Hu	96T	$458		Add to Cart

Human Spondin-1 - SPON1 ELISA Kit

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