Human SPOCK1/Testican-1 ELISA Kit PicoKine®

Mfr.No.

EK1848

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.027	0.075	0.141	0.272	0.477	0.899	1.591	1.973

Description

Human SPOCK1/Testican-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPOCK1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Testican-1; Protein SPOCK; SPOCK1; SPOCK; TIC1; TICN1

Immunogen

Expression system for standard: NS0; Immunogen sequence: R22-W439

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SPOCK1/Testican-1

Subcellular Localization

Extracellular matrix.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	21	67	435	26	73	474
Standard deviation	0.95	2.88	22.19	1.72	5.04	34.60
CV (%)	4.5%	4.3%	5.1%	6.6%	6.9%	7.3%

Protein Function

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Background

Testican-1 is a protein that in humans is encoded by the SPOCK1 gene. It is mapped to 5q31.2. This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	21	23	22	20	21	1.11	5.2%
Sample 2	67	71	74	66	69	3.20	4.6%
Sample 3	435	409	375	396	403	21.74	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6695
Antigen	SPOCK1/Testican-1
Gene Name	SPOCK1
UniProt ID	Q08629

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001613	EK1848	96 wells/kit	$750		Add to Cart

Human SPOCK1/Testican-1 ELISA Kit PicoKine®

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