Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

Mfr.No.

EK1501

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	200000000	20000000	2000000	200000	20000
O.D.	0.013	0.062	0.125	0.223	0.465	0.841	1.547	2.216

Description

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPHK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sphingosine kinase 1;SK 1;SPK 1;2.7.1.91;SPHK1;SPHK, SPK;

Molecular Weight

42518 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L384

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SPHK1

Subcellular Localization

Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	530	1744	8630	561	1890	8353
Standard deviation	25.44	104.64	576.1%%1	35.34	114.8%9	651.53
CV (%)	4.8%	6%	6.7%	6.1%%	6.1%	7.8%

Protein Function

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. .

Background

Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	530	551	501	466	512	31.94	6.2%
Sample 2	1744	1935	1605	1844	1782	122.5	6.8%
Sample 3	8630	9352	9453	8421	8964	446.11	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8877
Antigen	SPHK1/Sphingosine
Gene Name	SPHK1
UniProt ID	Q9NYA1
Protein Name	Sphingosine kinase 1
Tissue Specificity	Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. .

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,G Protein Signaling,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipid Signaling,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001095	EK1501	96 wells/kit	$750		Add to Cart

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

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