Human Sphingosine 1-phosphate Receptor Type 3

Mfr.No.

E6217Hu

Alternative Names

Endothelial differentiation G-protein coupled receptor 3;S1P receptor 3;S1P receptor Edg-3;S1P3;S1PR3;Sphingosine 1-phosphate receptor 3;Sphingosine 1-phosphate receptor Edg-3

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42250

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.66ng/L

Background

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

Gene ID	1903
UniProt ID	Q99500
Antigen	S1PR3

Research Area

Signal Transduction

Protocol

These standard curves of E6217Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.039	2.17	2.117
	2.3
800ng/L	1.481	1.409	1.356
	1.337
400ng/L	0.765	0.821	0.768
	0.877
200ng/L	0.493	0.514	0.462
	0.536
100ng/L	0.22	0.23	0.178
	0.241
0ng/L	0.07	0.052	0
	0.035

Precision

We measured random samples of E6217Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1211	46.28	3.8
Intra-Assay	2	18	204.3	9.69	4.7
Intra-Assay	3	18	454.8	27.56	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107579	E6217Hu	48T	$320		Add to Cart
BHE12107579	E6217Hu	96T	$458		Add to Cart

Human Sphingosine 1-phosphate Receptor Type 3 - S1P3 ELISA Kit

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