Human SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

Mfr.No.

EK1309

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	94	188	375	750	1500	3000
O.D.	0.010	0.072	0.139	0.253	0.481	0.842	1.437	2.327

Description

Human SPARCL1/Sparc Like 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPARCL1 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

SPARC-like protein 1;High endothelial venule protein;Hevin;MAST 9;SPARCL1;

Molecular Weight

75208 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-F664

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SPARCL1

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	81	322	1261	78	330	1329
Standard deviation	4.45	17.71	79.44	4.75	22.11	94.35
CV (%)	5.5%	5.5%	6.7%	6.1%	6.7%	7.1%

Background

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	81	81	84	89	83	3.26	3.9%
Sample 2	322	287	303	283	298	15.36	5.1%
Sample 3	1261	1280	1392	1332	1316	50.87	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8404
Gene Name	SPARCL1
UniProt ID	Q14515
Protein Name	SPARC-like protein 1
Tissue Specificity	Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Sequence	Belongs to the SPARC family.

Applications	ELISA
Research Area	Cancer,Oncoproteins/Suppressors,Tumor Biomarkers,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000919	EK1309	96 wells/kit	$499		Add to Cart

Human SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

Rate it now..!!