Human SPARC ELISA Kit PicoKine®

Mfr.No.

EK1210

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.099	0.169	0.251	0.388	0.638	1.020	1.446	1.931

Description

Human SPARC ELISA Kit PicoKine® (96 Tests). Quantitate Human SPARC in cell culture supeatants, serum and human milk. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

SPARC;Basement-membrane protein 40;BM-40;Osteonectin;ON;Secreted protein acidic and rich in cysteine;SPARC;ON;

Molecular Weight

34632 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-I303

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SPARC

Subcellular Localization

Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.

Sample Type

Cell culture supeatants, Serum, Human milk

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1247	5311	21553	1248	5203	22753
Standard deviation	93.52	393.01	1012.99	99.84	457.86	1296.92
CV (%)	7.5%	7.4%	4.7%	8%	8.8%	5.7%

Protein Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Background

SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1247	1275	1224	1339	1271	43.08	3.3%
Sample 2	5311	5517	5748	5864	5610	213.07	3.7%
Sample 3	21553	20541	21138	20396	20907	465.22	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6678
Gene Name	SPARC
UniProt ID	P09486
Protein Name	SPARC
Sequence	Belongs to the SPARC family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Lineage Markers,Lineage Specification,Mesenchymal Stem Cells,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Stem Cells,Structures,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000827	EK1210	96 wells/kit	$499		Add to Cart

Human SPARC ELISA Kit PicoKine®

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