Matrices listed below were spiked with certain level of SPAG1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPAG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
92
EDTA plasma(n=5)
88-105
95
heparin plasma(n=5)
89-92
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPAG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SPAG1/HSD-3.8/Infertility-related sperm protein Spag-1
Notes
For Research Use Only
UniProt ID
Q07617
Antigen
SPAG1
Applications
ELISA
Applications Notes
SPAG1 ELISA Kit allows for the in vitro quantitative determination of SPAG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SPAG1(Sperm-associated antigen 1) ELISA Kit