Human soluble Cluster of differentiation 100(sCD100)ELISA kit

Human soluble Cluster of differentiation 100(sCD100)ELISA kit

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Mfr.No.

CSB-E15792h

Alternative Names

sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, XXyac-YM68G7.1, C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, SEMAJ, coll-4, OTTHUMP00000021622, OTTHUMP00000208950, OTTHUMP00000208951, OTTHUMP00000208957, se

Reactivity

Human

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of guinea pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-104
EDTA plasma (n=4)	96	84-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:10	Average %	99
	Range %	95-103
1:20	Average %	94
	Range %	91-98
1:40	Average %	95
	Range %	90-100
1:80	Average %	92
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
1000	0.139	0.141	0.140
500	0.300	0.312	0.306
250	0.600	0.604	0.602
125	0.869	0.872	0.871
62.5	1.372	1.395	1.384
0	2.916	2.709	2.813

Species

Homo sapiens

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q92854
Antigen	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D

Applications

ELISA

Research Area

SEMA4D

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504366	CSB-E15792h	96T	$695	Add to Cart
BHE10504366	CSB-E15792h	5x96T	$3336	Add to Cart
BHE10504366	CSB-E15792h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.