Human soluble CD6 ELISA Kit PicoKine®

Mfr.No.

EK1221

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.002	0.056	0.108	0.219	0.416	0.728	1.202	1.845

Description

Human soluble CD6 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

T-cell differentiation antigen CD6;T12;TP120;CD6;CD6;

Molecular Weight

71801 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: H18-E398

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD6

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	255	1450	4911	250	1511	5268
Standard deviation	14.79	72.5	378.14	16.5	75.55	447.78
CV (%)	5.8%	5%	7.7%	6.6%	5%	8.5%

Protein Function

Involved in cell adhesion. Binds to CD166.

Background

CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	255	257	218	221	237	18.29	7.7%
Sample 2	1450	1421	1416	1269	1389	70.48	5%
Sample 3	4911	4340	4503	4742	4624	218.85	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	923
Gene Name	CD6
UniProt ID	P30203
Protein Name	T-cell differentiation antigen CD6
Tissue Specificity	Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

Applications	ELISA
Research Area	Adaptive Immunity,Hematopoietic Progenitors,Immunology,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000833	EK1221	96 wells/kit	$499		Add to Cart

Human soluble CD6 ELISA Kit PicoKine®

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