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Human Smoothened Homolog - SMO ELISA Kit

BHE12107547

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed: 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

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Gene ID 6608
UniProt ID Q99835
Antigen SMO
Research Area Others
Protocol These standard curves of E6185Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.167 2.156 2.104
2.145
800ng/L 1.68 1.609 1.557
1.538
400ng/L 0.967 0.974 0.922
0.981
200ng/L 0.669 0.691 0.639
0.714
100ng/L 0.403 0.401 0.348
0.398
0ng/L 0.059 0.052 0
0.045
Precision We measured random samples of E6185Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1086.6 61.8 5.7
Intra-Assay 2 18 107.6 6.52 6.1
Intra-Assay 3 18 478.8 32.78 6.8
Storage 2-8°C
SKU Supplier No. Size Your price Quantity
BHE12107547 E6185Hu 48T $320
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BHE12107547 E6185Hu 96T $458
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