Human Smad Nuclear-interacting Protein 1

Mfr.No.

E6180Hu

Alternative Names

FHA domain-containing protein SNIP1;PML1;PMRED;Smad nuclear-interacting protein 1;SNIP1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

45778

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.78ng/L

Background

Required for pre-mRNA splicing as component of the spliceosome (PubMed:29360106). Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

Gene ID	79753
UniProt ID	Q8TAD8
Antigen	SNIP1

Research Area

Signal Transduction; Epigenetics and Nuclear Signaling

Protocol

These standard curves of E6180Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.83	1.899	1.869
	1.967
1200ng/L	0.949	1.018	0.988
	1.086
600ng/L	0.561	0.538	0.508
	0.515
300ng/L	0.184	0.21	0.18
	0.235
150ng/L	0.117	0.134	0.104
	0.152
0ng/L	0.029	0.03	0
	0.031

Precision

We measured random samples of E6180Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1385.55	80.23	5.79
Intra-Assay	2	18	1489.54	42.42	2.85
Intra-Assay	3	18	556.73	29.07	5.22

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107542	E6180Hu	48T	$320		Add to Cart
BHE12107542	E6180Hu	96T	$458		Add to Cart

Human Smad Nuclear-interacting Protein 1 - SNIP1 ELISA Kit

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