Matrices listed below were spiked with certain level of SM/SPH and the recovery rates were calculated by comparing the measured value to the expected amount of SM/SPH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
93
EDTA plasma(n=5)
92-104
97
heparin plasma(n=5)
89-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SM/SPH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SM/SPH ELISA Kit allows for the in vitro quantitative determination of SM/SPH concentrations in serum, plasma, tissue homogenates and other biological fluids.