Human Ski Oncogene - SKI ELISA Kit

Mfr.No.

E6177Hu

Alternative Names

Proto-oncogene c-Ski;SGS;SKI;Ski oncogene;SKV

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

80005

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.02ng/L

Background

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

Gene ID	6497
UniProt ID	P12755
Antigen	SKI

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E6177Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.094	2.096	2.047
	2.097
1200ng/L	0.941	1.035	0.986
	1.129
600ng/L	0.48	0.463	0.414
	0.445
300ng/L	0.277	0.254	0.206
	0.232
150ng/L	0.155	0.16	0.111
	0.165
0ng/L	0.057	0.049	0
	0.041

Precision

We measured random samples of E6177Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	876.39	38.07	4.34
Intra-Assay	2	18	1993.09	34.11	1.71
Intra-Assay	3	18	1379.31	49.57	3.59

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107539	E6177Hu	48T	$320		Add to Cart
BHE12107539	E6177Hu	96T	$458		Add to Cart

Human Ski Oncogene - SKI ELISA Kit

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