Human Siglec-7/CD328 ELISA Kit PicoKine®

Mfr.No.

EK1508

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.012	0.153	0.231	0.461	0.844	1.298	1.645	2.124

Description

Human Siglec-7/CD328 ELISA Kit PicoKine® (96 Tests). Quantitate Human SIGLEC7 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sialic acid-binding Ig-like lectin 7;Siglec-7;Adhesion inhibitory receptor molecule 1;AIRM-1;CDw328;D-siglec;QA79 membrane protein;p75;CD328;SIGLEC7;AIRM1;

Molecular Weight

51143 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-L353

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Siglec-7

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	174	1303	3179	179	1384	3570
Standard deviation	7.83	93.81	238.43	8.41	123.17	224.91
CV (%)	4.5%	7.2%	7.5%	4.7%	8.9%	6.3%

Protein Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase (s) via their SH2 domain (s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). .

Background

Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	174	199	198	204	193	11.62	6%
Sample 2	1303	1333	1368	1214	1304	57.08	4.3%
Sample 3	3795	3634	4070	3787	3821	157.16	4.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	27036
Gene Name	SIGLEC7
UniProt ID	Q9Y286
Protein Name	Sialic acid-binding Ig-like lectin 7
Tissue Specificity	Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression found in placenta, liver, lung, spleen, and peripheral blood leukocytes.

Applications	ELISA
Research Area	Cardiovascular,Cell Type Marker,Hematopoietic Progenitors,Immunology,Innate Immunity,Neuron Marker,Neuroscience,NK Cells,Stem Cells,Surface Molecules

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001090	EK1508	96 wells/kit	$499		Add to Cart

Human Siglec-7/CD328 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Siglec-7/CD328 ELISA Kit PicoKine®

Rate it now..!!