Human Sh3Kbp1 Binding Protein 1

Mfr.No.

E7316Hu

Alternative Names

SETA-binding protein 1;SH3KBP1-binding protein 1;SHKBP 1;SHKBP1

Reactivity

Human

Detection Range

20-3800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

76344

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

12.14ng/L

Background

Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.

Gene ID	92799
UniProt ID	Q8TBC3
Antigen	SHKBP1

Research Area

Neuroscience

Protocol

These standard curves of E7316Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.243	2.19	2.145
	2.137
1000ng/L	1.385	1.313	1.268
	1.242
500ng/L	0.766	0.81	0.765
	0.854
250ng/L	0.482	0.506	0.461
	0.529
125ng/L	0.191	0.187	0.142
	0.184
0ng/L	0.056	0.045	0
	0.035

Precision

We measured random samples of E7316Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1423.1	55.03	3.9
Intra-Assay	2	18	799.6	46.69	5.8
Intra-Assay	3	18	244.2	16.92	6.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115530	E7316Hu	48T	$320		Add to Cart
BHE12115530	E7316Hu	96T	$458		Add to Cart

Human Sh3Kbp1 Binding Protein 1 - SHKBP1 ELISA Kit

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