Human SFRP5/Sarp3 ELISA Kit PicoKine®

Mfr.No.

EK1472

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.087	0.149	0.216	0.390	0.789	1.226	1.896	2.419

Description

Human SFRP5/Sarp3 ELISA Kit PicoKine® (96 Tests). Quantitate Human SFRP5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Secreted frizzled-related protein 5;sFRP-5;Frizzled-related protein 1b;FRP-1b;Secreted apoptosis-related protein 3;SARP-3;SFRP5;FRP1B, SARP3;

Molecular Weight

35563 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: E30-H317

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SFRP5

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	329	1068	4969	309	1159	5218
Standard deviation	20.39	69.42	203.72	21.93	96.19	250.46
CV (%)	6.2%	6.5%	4.1%	7.1%	8.3%	4.8%

Protein Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Background

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	329	353	327	347	339	11.22	3.3%
Sample 2	1068	1216	1033	1211	1132	82.45	7.2%
Sample 3	4969	5003	5645	5635	5313	327.23	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6425
Gene Name	SFRP5
UniProt ID	Q5T4F7
Protein Name	Secreted frizzled-related protein 5
Tissue Specificity	Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle. .

Applications	ELISA
Research Area	Secreted,Signaling Pathways,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001072	EK1472	96 wells/kit	$750		Add to Cart

Human SFRP5/Sarp3 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human SFRP5/Sarp3 ELISA Kit PicoKine®

Rate it now..!!