Matrices listed below were spiked with certain level of SERPINA4 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINA4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
97
EDTA plasma(n=5)
88-105
94
heparin plasma(n=5)
88-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SERPINA4 ELISA Kit allows for the in vitro quantitative determination of SERPINA4 concentrations in serum, plasma, tissue homogenates and other biological fluids.