Human Serpin A6 ELISA Kit PicoKine®

Mfr.No.

EK1963

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.033	0.107	0.176	0.228	0.356	0.620	1.091	1.987

Description

Human Serpin A6 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA6 in cell culture supeatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Corticosteroid-binding globulin; CBG; Serpin A6; Transcortin; SERPINA6; CBG

Immunogen

Expression system for standard: NS0; Immunogen sequence: M23-V405

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Serpin A6

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2728	12106	43028	2986	12333	47148
Standard deviation	141.86	544.77	2452.60	206.03	715.31	2970.32
CV (%)	5.2%	4.5%	5.7%	6.9%	5.8%	6.3%

Protein Function

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.

Background

Corticosteroid-binding globulin (CBG), also known as Serpin A6 is a protein that in humans is encoded by the SERPINA6 gene. It is mapped to 14q32.13. This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2728	3062	2675	2954	2854	159.04	5.5%
Sample 2	12106	13177	12457	12922	12665	413.50	3.2%
Sample 3	43028	43427	45382	41055	43223	1536.32	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	866
Gene Name	SERPINA6
UniProt ID	P08185
Tissue Specificity	Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.

Applications	ELISA
Research Area	Cancer,Lipid Metabolism,Metabolism,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001470	EK1963	96 wells/kit	$750		Add to Cart

Human Serpin A6 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Serpin A6 ELISA Kit PicoKine®

Rate it now..!!