Human Serpin A12 ELISA Kit PicoKine®

Mfr.No.

EK1874

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.025	0.160	0.263	0.491	0.852	1.290	1.878	2.305

Description

Human Serpin A12 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA12 in cell culture supeatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-K414

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Serpin A12

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	212	1109	5465	232	1235	6154
Standard deviation	11.24	68.76	245.93	10.90	79.04	332.32
CV (%)	5.3%	6.2%	4.5%	4.7%	6.4%	5.4%

Protein Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Background

This gene is mapped to 14q32.13.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	212	215	232	220	219	7.62	3.4%
Sample 2	1109	1215	1235	1149	1177	50.53	4.2%
Sample 3	5465	6356	5987	5976	5946	317.03	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	145264
Gene Name	SERPINA12
UniProt ID	Q8IW75
Tissue Specificity	Expressed in visceral adipose tissues.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Diabetes,Diabetes-associated,Growth Factors/Hormones,Heart Disease,Metabolism,Neurology Process,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001492	EK1874	96 wells/kit	$499		Add to Cart

Human Serpin A12 ELISA Kit PicoKine®

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