Human Serine - Threonine-protein Kinase Nek3

Mfr.No.

E3202Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HSPK 36;NEK 3;NEK3;Never in mitosis A-related kinase 3;NimA-related protein kinase 3;Serine/threonine-protein kinase Nek3

Molecular Weight

57705

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3202Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.7	0.09	5.4
Intra-Assay	2	18	3.9	0.22	5.8
Intra-Assay	3	18	6.4	0.21	3.3

Background

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

Protocol

These standard curves of E3202Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.565	2.525	2.483
	2.486
6ng/ml	1.592	1.497	1.455
	1.403
3ng/ml	0.88	0.881	0.838
	0.882
1.5ng/ml	0.51	0.465	0.422
	0.419
0.75ng/ml	0.23	0.226	0.183
	0.222
0ng/ml	0.04	0.043	0
	0.046

Gene ID	4752
UniProt ID	P51956

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104731	E3202Hu	48T	$320		Add to Cart
BHE12104731	E3202Hu	96T	$458		Add to Cart

Human Serine - Threonine-protein Kinase Nek3 - NEK3 ELISA Kit

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