Human Serine Threonine-protein Kinase B-raf

Mfr.No.

E3906Hu

Description

This sandwich kit is for the accurate quantitative detection of human Serine, Threonine-protein Kinase B-raf (also known as BRAF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BRAF;B-raf;BRAF1;B-RAF1;NS7;p94;Proto-oncogene B-Raf;RAFB1;Serine/threonine-protein kinase B-raf;v-Raf murine sarcoma viral oncogene homolog B1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

84437

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.11ng/L

Background

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

Gene ID	673
UniProt ID	P15056
Antigen	BRAF

Research Area

Cell Biology

Protocol

These standard curves of E3906Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.171	2.287	2.231
	2.404
1200ng/L	1.313	1.352	1.296
	1.392
600ng/L	0.832	0.8	0.744
	0.769
300ng/L	0.487	0.474	0.418
	0.462
150ng/L	0.314	0.319	0.263
	0.325
0ng/L	0.073	0.056	0
	0.04

Precision

We measured random samples of E3906Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1170.07	42	3.59
Intra-Assay	2	18	491.29	39.47	8.03
Intra-Assay	3	18	980.84	53.92	5.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105344	E3906Hu	48T	$320		Add to Cart
BHE12105344	E3906Hu	96T	$458		Add to Cart

Human Serine Threonine-protein Kinase B-raf - BRAF ELISA Kit

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