Human Serglycin - SRGN ELISA Kit

Mfr.No.

E4167hu

Sensitivity

0.043ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Serglycin (also known as SRGN) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Hematopoietic proteoglycan core protein;P.PG;Platelet proteoglycan core protein;Secretory granule proteoglycan core protein;Serglycin;SRGN

Molecular Weight

17652

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4167hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	13.2	0.63	4.8
Intra-Assay	2	18	6.9	0.36	5.2
Intra-Assay	3	18	3.4	0.16	4.9

Background

Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.

Protocol

These standard curves of E4167hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.068	2.157	2.118
	2.246
12ng/ml	1.364	1.462	1.423
	1.56
6ng/ml	0.764	0.765	0.726
	0.767
3ng/ml	0.456	0.459	0.419
	0.462
1.5ng/ml	0.125	0.152	0.113
	0.179
0ng/ml	0.05	0.039	0
	0.028

Gene ID	5552
UniProt ID	P10124

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105568	E4167hu	48T	$320		Add to Cart
BHE12105568	E4167hu	96T	$458		Add to Cart

Human Serglycin - SRGN ELISA Kit

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