Human Semaphorin 7A - SEMA7A ELISA Kit

Mfr.No.

E2538Hu

Sensitivity

0.046ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Semaphorin 7A (also known as SEMA7A) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD108;CDw108;JMH blood group antigen;John-Milton-Hargen human blood group Ag;Sema K1;Sema L;SEMA7A;Semaphorin-7A;Semaphorin-K1;Semaphorin-L

Molecular Weight

74824

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2538Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.2	0.38	5.2
Intra-Assay	2	18	15.3	0.38	2.5
Intra-Assay	3	18	4.9	0.3	6.1

Background

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

Protocol

These standard curves of E2538Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.166	2.256	2.203
	2.347
12ng/ml	1.188	1.194	1.141
	1.2
6ng/ml	0.677	0.746	0.693
	0.816
3ng/ml	0.398	0.436	0.383
	0.475
1.5ng/ml	0.191	0.194	0.141
	0.198
0ng/ml	0.058	0.053	0
	0.049

Gene ID	8482
UniProt ID	O75326

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104076	E2538Hu	48T	$320		Add to Cart
BHE12104076	E2538Hu	96T	$458		Add to Cart

Human Semaphorin 7A - SEMA7A ELISA Kit

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