Human Semaphorin 6B - SEMA6B ELISA Kit

Mfr.No.

E1325Hu

Alternative Names

SEMA 6B;Sema Z;SEMA6B;Semaphorin-6B;Semaphorin-Z

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

95285

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.039ng/ml

Background

Functions as a cell surface repellent for mossy fibers of developping neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons. (Microbial infection) Acts as a receptor for P.sordellii toxin TcsL in the in the vascular endothelium.

Gene ID	10501
UniProt ID	Q9H3T3
Antigen	SEMA6B

Research Area

Others

Protocol

These standard curves of E1325Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.178	2.157	2.123
	2.137
12ng/ml	1.275	1.237	1.202
	1.199
6ng/ml	0.761	0.798	0.763
	0.835
3ng/ml	0.452	0.457	0.422
	0.462
1.5ng/ml	0.229	0.2	0.165
	0.17
0ng/ml	0.031	0.035	0
	0.038

Precision

We measured random samples of E1325Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.4	0.54	5.7
Intra-Assay	2	18	17.4	0.45	2.6
Intra-Assay	3	18	2.2	0.16	7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102967	E1325Hu	48T	$320		Add to Cart
BHE12102967	E1325Hu	96T	$458		Add to Cart

Human Semaphorin 6B - SEMA6B ELISA Kit

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