Human Semaphorin 3D - SEMA3D ELISA Kit

Mfr.No.

E1512Hu

Sensitivity

0.052ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Semaphorin 3D (also known as Sema3d) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

SEMA3D;Semaphorin-3D

Molecular Weight

89651

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1512Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.2	0.1	4.7
Intra-Assay	2	18	4.3	0.27	6.3
Intra-Assay	3	18	7.2	0.3	4.2

Background

Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).

Protocol

These standard curves of E1512Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.358	2.232	2.184
	2.105
12ng/ml	1.77	1.804	1.756
	1.838
6ng/ml	1.134	1.135	1.088
	1.137
3ng/ml	0.621	0.653	0.605
	0.685
1.5ng/ml	0.393	0.395	0.348
	0.398
0ng/ml	0.035	0.048	0
	0.061

Gene ID	223117
UniProt ID	O95025

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103127	E1512Hu	48T	$320		Add to Cart
BHE12103127	E1512Hu	96T	$458		Add to Cart

Human Semaphorin 3D - SEMA3D ELISA Kit

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